Product Description for AKTIP / FTS
Rabbit anti Canine, Chicken, Human, Mouse, Rat, Zebrafish, African clawed frog AKTIP / FTS.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Properties for AKTIP / FTS
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | AKT-interacting protein, FT1, Fused toes protein homolog |
| Presentation | Aff - Purified |
| Reactivity | African clawed frog, Can, Chk, Hu, Ms, Rt, Ze |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Western blotting (0.2 - 1.0 µg/ml) |
| Background | AKTIP is the component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). AKTIP regulates apoptosis by enhancing phosphorylation and activation of AKT1. AKTIP increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade.The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein. |
| General Readings | "Regulation of apoptosis by the Ft1 protein, a new modulator of protein kinase B/Akt." Remy I., Michnick S.W. Mol. Cell. Biol. 24:1493-1504(2004) "An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex." Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W. Mol. Biol. Cell 19:5059-5071(2008) |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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| Gene ID | 64400 |
Accessory Products
Proteins and/or Positive Controls
Proteins for AKTIP / FTS (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP315277 |
AKTIP / FTS (transcript variant 2) |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| H00064400-P01-10 |
AKTIP / FTS |
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|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00064400-P01-25 |
AKTIP / FTS |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for AKTIP / FTS (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00064400-T01 |
FTS 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| NBL1-07445 |
FTS Lysate |
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![Western Blot: FTS Lysate [NBL1-07445] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for AKTIP](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/FTS%20Lysate-Western%20Blot-NBL1-07445-img0001.jpg) |
Protein | ||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for AKTIP / FTS (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
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|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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