discontinued

AP42132PU-N TSFM antibody

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Rabbit anti Bovine, Canine, Human, Mouse, Rat, Zebrafish, African clawed frog TSFM

AP42132PU-N

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  • AP42132PU-N

Product Description for TSFM

Rabbit anti Bovine, Canine, Human, Mouse, Rat, Zebrafish, African clawed frog TSFM.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for TSFM

Product Category Primary Antibodies
Quantity 50 µg
Synonyms EF-Ts, EF-TsMt, Elongation factor Ts mitochondrial
Presentation Aff - Purified
Reactivity African clawed frog, Bov, Can, Hu, Ms, Rt, Ze
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P43897
Immunogen:
The immunogen for anti-TSFM antibody: synthetic peptide directed towards the N terminal of human TSFM.
AA Sequence:
KQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCET
GeneID:
10102
Application Western blotting (0.2 - 1.0 µg/ml)
Immunohistochemsitry on paraffin embedded sections (2 - 8 µg/ml)
Background The TSFM gene encodes a protein that is expressed in all tissues, with the highest levels of expression in skeletal muscle, liver, and kidney. It associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Defects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes
General Readings Vernon,J.L., et al., (2000) Cytogenet. Cell Genet. 89 (3-4), 145-146
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Purified using peptide immunoaffinity column
State:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat, Dog, African clawed frog, Bovine, Zebrafish
Species reactivity (tested):
Human
Gene ID 10102

Accessory Products

Proteins and/or Positive Controls

Proteins for TSFM (9 products)

Catalog No. Species Pres. Purity   Source  

TSFM (46-346, )

TSFM Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

TSFM (46-346, )

TSFM Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

TSFM

TSFM Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

TSFM

TSFM Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

TSFM

TSFM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TSFM

TSFM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TSFM

TSFM Human Purified
  Abnova Taiwan Corp.

TSFM

TSFM Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

TSFM

TSFM Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for TSFM (3 products)

Catalog No. Species Pres. Purity   Source  

TSFM Lysate(Denatured)

TSFM Lysate(Denatured)
  Abnova Taiwan Corp.

TSFM Lysate

Western Blot: TSFM Lysate [NBL1-17357] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for TSFM
  Novus Biologicals Inc.

TSFM overexpression lysate

TSFM overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.
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