Product Description for TSFM
Rabbit anti Bovine, Canine, Human, Mouse, Rat, Zebrafish, African clawed frog TSFM.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Properties for TSFM
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | EF-Ts, EF-TsMt, Elongation factor Ts mitochondrial |
| Presentation | Aff - Purified |
| Reactivity | African clawed frog, Bov, Can, Hu, Ms, Rt, Ze |
| Applications | P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Western blotting (0.2 - 1.0 µg/ml) Immunohistochemsitry on paraffin embedded sections (2 - 8 µg/ml) |
| Background | The TSFM gene encodes a protein that is expressed in all tissues, with the highest levels of expression in skeletal muscle, liver, and kidney. It associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Defects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes |
| General Readings | Vernon,J.L., et al., (2000) Cytogenet. Cell Genet. 89 (3-4), 145-146 |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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| Gene ID | 10102 |
Accessory Products
Proteins and/or Positive Controls
Proteins for TSFM (9 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR51511PU-N |
TSFM (46-346, ) |
||||||
|
Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.5 mg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR51511PU-S |
TSFM (46-346, ) |
||||||
|
Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.1 mg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| H00010102-P01-10 |
TSFM |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010102-P01-25 |
TSFM |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010102-Q01-10 |
TSFM |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010102-Q01-25 |
TSFM |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010102-Q02 |
TSFM |
||||||
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00010102-Q02-10 |
TSFM |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010102-Q02-25 |
TSFM |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for TSFM (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00010102-T01 |
TSFM Lysate(Denatured) |
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| Abnova Taiwan Corp. | |||||||
| NBL1-17357 |
TSFM Lysate |
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| Novus Biologicals Inc. | |||||||
| LY432920 |
TSFM overexpression lysate |
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0.1 mg /
€315.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for TSFM (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
![Western Blot: TSFM Lysate [NBL1-17357] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for TSFM](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/TSFM%20Lysate-Western%20Blot-NBL1-17357-img0001.jpg)
