Nitrotyrosine - AM26352BT-N | acris-antibodies.com

Product Description for Nitrotyrosine

Mouse anti Nitrotyrosine HM.11.
Presentation: Biotin
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay, Paraffin Sections, Frozen Sections.

Properties for Nitrotyrosine

Product Category	Primary Antibodies
Quantity	50 µg
Synonyms	NO-Tyrosine, Nitro-Tyrosine
Presentation	Biotin
Applications	C, E, P, WB
Clonality	Monoclonal
Clone	HM.11
Host	Mouse
Isotype	IgG2b
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: Nitrated KLH
Isotype control	SM12B
Application	Immunohistochemistry on frozen sections (1,3): Also cytospins: acetone fixation 10 min -20 °C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); blocking with 10% NGS or 5 % BSA for 30 min. The typical starting working dilution is 1:10. Immunohistochemistry on paraffin sections (4,5): 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100 °C as antigen retrieval treatment. Positive control: Human lung tissue. The typical starting working dilution is 1:10. Immunoassays (1). Western blot (2-5): Reduced and no-reduced samples; block with 5% BSA or skimmed milk. Positive control: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate. The typical starting working dilution is 1:10.
Background	Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage.
Caution	Keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody!
Concentration	0.1 mg/ml
General Readings	ter Steege JC, Koster-Kamphuis L, van Straaten EA, Forget PP, Buurman WA. Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA. Free Radic Biol Med. 1998 Nov 15;25(8):953-63. PubMed PMID: 9840741. Beckmann JS, Ye YZ, Anderson PG, Chen J, Accavitti MA, Tarpey MM, et al. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol Chem Hoppe Seyler. 1994 Feb;375(2):81-8. PubMed PMID: 8192861. Kooy NW, Royall JA, Ye YZ, Kelly DR, Beckman JS. Evidence for in vivo peroxynitrite production in human acute lung injury. Am J Respir Crit Care Med. 1995 Apr;151(4):1250-4. PubMed PMID: 7697261. Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1620-4. PubMed PMID: 2154753. (Free PMC Article available)
Storage	Store at 2 - 8 °C. Shelf life: one year from despatch.
Format	Label: Biotin Purification: Protein G Buffer System: PBS Stabilizers: 0.1% bovine serum albumin State: Liquid 0.2 µm filtered Ig fraction
Specificity	Specificity: The monoclonal antibody HM.11 recognizes modified amino acid nitrotyrosine in all different species. It recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. Does not cross react with Phosphotyrosine or Chlorotyrosine.

Accessory Products

Recommended Secondary Antibodies for Nitrotyrosine (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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AM26352BT-N Nitrotyrosine antibody

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Product Description for Nitrotyrosine

Properties for Nitrotyrosine

Datasheet Extract

Accessory Products

Recommended Secondary Antibodies for Nitrotyrosine (11 products)

Mouse IgG (H+L chain) antibody

Mouse IgG (H+L chain) antibody

Mouse IgG (H+L chain) antibody

Mouse IgG (H+L chain) antibody

Mouse IgG (H+L chain) antibody

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody

Mouse IgG (H+L chain), adsorbed antibody

Mouse IgG (H+L chain), adsorbed antibody

Mouse IgG (H+L chain), adsorbed antibody

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