Product Description for FGF basic / FGF2
FGF basic / FGF2.
Presentation: Purified
Presentation: Purified
Properties for FGF basic / FGF2
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 50 µg |
| Synonyms | BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2 |
| Presentation | Purified |
| Molecular weight | 16.5 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 95 % by SDS - PAGE |
| Background | Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Protocols | 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast )2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul)5. Incubation time : 24 hr (after sample treatment)6. Concentration range: 10 pg/ml - 1 ug/ml 7. Detection method : BrdU assay |
| General Readings | Nakayama T., et al. (2007) Blood. 109(2):1363-72.A E Eriksson., et al. (1991) PNAS. 88: 3441-3445 |
| Storage | Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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