Cardiac Troponin C (1-161, His-tag) - AR50085PU-N

Product Description for Cardiac Troponin C

Cardiac Troponin C.
Presentation: Purified

Immunogen	Swiss Prot Num: P63316
Purity	> 95 % by SDS - PAGE
Background	Troponin C type 1, also known as TNNC1, is a member of troponins family. Troponin has 3 subunits, Troponin I(Tn-I), Troponin T(Tn-T) and Troponin C(Tn-C). Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. TNNC1 is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers.
Concentration	1 mg/ml (determined by Bradford assay)
General Readings	Wu A H)., et al. (1998) Clin Chem. 44:1198-1208.Hamm C W., et al. (2001) Thromb Res. 103:S63-S69.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human TNNC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E` Molecular weight: (181aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl Purity: >95% by SDS - PAGE State: Liquid purified protein Purified