MED20 (1-212, His-tag) - AR50679PU-N

Product Description for MED20

MED20.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	Mediator complex 20, Mediator of RNA polymerase II transcription subunit 20, TRF-proximal protein homolog, TRFP
Presentation	Purified
Molecular weight	25 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: Q9H944
Purity	> 90 % by SDS - PAGE
Background	MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors.
Concentration	0.25 mg/ml (determined by Bradford assay)
General Readings	Sato S, Tomomori-Sato C, et al. (2004). Mol Cell. 14(5):685-91.Zhang X, Krutchinsky A, et al. (2011). Mol Cell. 19(1):89-100.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human MED20 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR` Molecular weight: (235aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 1mM DTT Purity: >90% by SDS - PAGE State: Liquid purified protein Purified