AR50684PU-N MPST (1-297, His-tag)

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0.5 mg / €820.00
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Quick Overview

MPST

Product Description for MPST

MPST.
Presentation: Purified

Properties for MPST

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms 3-mercaptopyruvate sulfurtransferase, MST, TST2
Presentation Purified
Molecular weight 35 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P25325
Purity > 95 % by SDS - PAGE
Background MPST catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Billaut-Laden I, Rat E, et al. (2006). Toxicol Lett. 165(2):101-11.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human MPST protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMASPQL CRALVSAQWV AEALRAPRAG QPLQLLDASW YLPKLGRDAR REFEERHIPG AAFFDIDQCS DRTSPYDHML PGAEHFAEYA GRLGVGAATH VVIYDASDQG LYSAPRVWWM FRAFGHHAVS LLDGGLRHWL RQNLPLSSGK SQPAPAEFRA QLDPAFIKTY EDIKENLESR RFQVVDSRAT GRFRGTEPEP RDGIEPGHIP GTVNIPFTDF LSQEGLEKSP EEIRHLFQEK KVDLSKPLVA TCGSGVTACH VALGAYLCGK PDVPIYDGSW VEWYMRARPE DVISEGRGKT H
Molecular weight:
(321aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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