POLR2I (1-125, His-tag) - AR50652PU-N

Product Description for POLR2I

POLR2I.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.25 mg
Synonyms	DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, RNA polymerase II 14.5 kDa subunit, RNA polymerase II subunit B9, RPB14.5, RPB9
Presentation	Purified
Molecular weight	17.0 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P36954
Purity	> 85 % by SDS - PAGE
Background	POLR2I, also known as RPB9, belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. This protein is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Also it is synthesizes mRNA precursors and many functional non-coding RNAs.
Concentration	1 mg/ml (determined by Bradford assay)
General Readings	Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human PORL2I protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMEPDGTY EPGFVGIRFC QECNNMLYPK EDKENRILLY ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ KCGHKEAVFF QSHSARAEDA MRLYYVCTAP HCGHRWTE` Molecular weight: (148aa) confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT Purity: >85% by SDS - PAGE State: Liquid purified protein Purified