Product Description for Galectin-4
Galectin-4.
Presentation: Purified
Presentation: Purified
Properties for Galectin-4
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.25 mg |
| Synonyms | Antigen NY-CO-27, Galectin4, L-36 lactose-binding protein, L36LBP, LGALS4, Lactose-binding lectin 4 |
| Presentation | Purified |
| Molecular weight | 38.8 kDa |
| Source | E. coli |
| Species (Protein) | Mouse |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
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|---|---|
| Purity | > 90 % by SDS - PAGE |
| Background | Lgals4, also known as galectin4, belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions, which inhibits chronic inflammations, GVHD, and allergic reactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Protocols | Biological assay: 1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g, Sodium citric acid 0.8g, Citr ic acid 0.055 g, D - glucose 2.05g in DW100 ml) 2. Centrifuge at 15000rpm for 10 minutes and wash four times with PBS. 3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension. 4. Incubate for 1h at 37°C and wash four time s with PBS. 6 . Load 50 ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red cell - in - PBS in u shaped wells. 7 . Add 25 ul of serial diluted galectin protein in PBS to each well plate. (Round bot tom 96 well plate). 8 . Incubate for 30min at room temperature to observe visible agglutination. |
| General Readings | Huflejt ME., et al. (1997) J Biol Chem. 272(22):14294-303. Huflejt ME., et al. (2004) Glycoconj J. 20(4):247-55. |
| Storage | Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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