POLR2J (1-117, His-tag) - AR50598PU-S

Product Description for POLR2J

POLR2J.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	50 µg
Synonyms	DNA-directed RNA polymerase II subunit J-1, DNA-directed RNA polymerase II subunit RPB11-a, POLR2J1, RNA polymerase II 13.3 kDa subunit, RNA polymerase II subunit B11-a, RPB11a
Presentation	Purified
Molecular weight	15.7 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P52435
Purity	> 90 % by SDS - PAGE
Background	POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
Concentration	0.5 mg/ml (determined by Bradford assay)
General Readings	Proshkin,S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980Shpakovskii,D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE` Molecular weight: (140aa) confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, and 2mM EDTA Purity: >90% by SDS - PAGE State: Liquid purified protein Purified