AR50599PU-S NDUFAF1 (25-327, His-tag)

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0.1 mg / €400.00
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NDUFAF1

Product Description for NDUFAF1

NDUFAF1.
Presentation: Purified

Properties for NDUFAF1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms CGI-65, CIA30, Complex I intermediate-associated protein 30, mitochondrial, mitochondrial NDUFAF1
Presentation Purified
Molecular weight 37 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y375
Purity > 90 % by SDS - PAGE
Background NDUFAF1 is a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The protein is required for assembly of complex I, and mutations in this protein are a cause of mitochondrial complex I deficiency.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Vogel RO., et al. (2005). FEBS J. 272(20):5317-26.Janssen R., et al. (2002). Hum Genet. 110(3):264-70.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human NDUFAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSYPFLGIR FAEYSSSLQK PVASPGKASS QRKTEGDLQG DHQKEVALDI TSSEEKPDVS FDKAIRDEAI YHFRLLKDEI VDHWRGPEGH PLHEVLLEQA KVVWQFRGKE DLDKWTVTSD KTIGGRSEVF LKMGKNNQSA LLYGTLSSEA PQDGESTRSG YCAMISRIPR GAFERKMSYD WSQFNTLYLR VRGDGRPWMV NIKEDTDFFQ RTNQMYSYFM FTRGGPYWQE VKIPFSKFFF SNRGRIRDVQ HELPLDKISS IGFTLADKVD GPFFLEIDFI GVFTDPAHTE EFAYENSPEL NPRLFK
Molecular weight:
(326aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT, 2mM EDTA
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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