LIN28B (1-250, His-tag) - AR50578PU-N

Product Description for LIN28B

LIN28B.
Presentation: Purified

Immunogen	Swiss Prot Num: Q6ZN17
Purity	> 85 % by SDS - PAGE
Background	Lin28B belongs to the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B is acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor.
Concentration	0.25 mg/ml (determined by Bradford assay)
General Readings	Heo I., et al. (2008) Mol. Cell 32:276-284Richards M, et al. (2004) Stem Cells 22 (1): 51–64.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human Lin28B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT` Molecular weight: (273aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol,2mM DTT Purity: >85% by SDS - PAGE State: Liquid purified protein Purified