AR50592PU-N ASPRV1 / SASP (191-326, His-tag)

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0.5 mg / €820.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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ASPRV1 / SASP

Product Description for ASPRV1 / SASP

ASPRV1 / SASP.
Presentation: Purified

Properties for ASPRV1 / SASP

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms Retroviral-like aspartic protease 1, SASPase, Skin aspartic protease, Skin-specific retroviral-like aspartic protease, TAPS, TPA-inducible aspartic proteinase-like protein
Presentation Purified
Molecular weight 17.2 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q53RT3
Purity > 90 % by SDS - PAGE
Background ASPRV1, also known as MUNO, SASP and ASAPase, is a protein that contains 1 peptidase A2 domain. This protein is expressed primarily in the granular layer of the epidermis and inner root sheath of hair follicles and localized to membrane region. This protein undergoes autocleavage which is necessary for activation of the protein.
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings
  1. Matsui T, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, et al. SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing. EMBO Mol Med. 2011 Jun;3(6):320-33. doi: 10.1002/emmm.201100140. Epub 2011 May 3. PubMed PMID: 21542132. (Free PMC Article available, 8 images available)
  2. Rhiemeier V, Breitenbach U, Richter KH, Gebhardt C, Vogt I, Hartenstein B, et al. A novel aspartic proteinase-like gene expressed in stratified epithelia and squamous cell carcinoma of the skin. Am J Pathol. 2006 Apr;168(4):1354-64. PubMed PMID: 16565508. (Free PMC Article available, 8 images available)
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human ASPRV1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSSMGKGYY LKGKIGKVPV RFLVDSGAQV SVVHPNLWEE VTDGDLDTLQ PFENVVKVAN GAEMKILGVW DTAVSLGKLK LKAQFLVANA SAEEAIIGTD VLQDHNAILD FEHRTCTLKG KKFRLLPVGG SLEDEFDLE
Molecular weight:
(159aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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