POLR2J2 (1-115, His-tag) - AR50567PU-N

Product Description for POLR2J2

POLR2J2.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.25 mg
Synonyms	DNA-directed RNA polymerase II subunit J2, DNA-directed RNA polymerase II subunit RPB11-b1, RNA polymerase II subunit B11-b1, RPB11, RPB11b1
Presentation	Purified
Molecular weight	15.5 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: Q9GZM3
Purity	> 95 % by SDS - PAGE
Background	DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Concentration	0.5 mg/ml (determined by Bradford assay)
General Readings	Palangat M., et al. (2004) J Mol Biol. 341:429-442Uivari A., et al. (2006) Nat Struct Mol Biol. 13: 49-54.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP` Molecular weight: (138aa) confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol,1mM DTT Purity: >95% by SDS - PAGE State: Liquid purified protein Purified