AR50446PU-S DIMT1L (1-313, His-tag)

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0.1 mg / €400.00
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Quick Overview

DIMT1L

Product Description for DIMT1L

DIMT1L.
Presentation: Purified

Properties for DIMT1L

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms 18S rRNA dimethylase, DIM1 dimethyladenosine transferase 1-like, DIMT1, HUSSY-05, N'-adenosyl(rRNA) dimethyltransferase, S-adenosylmethionine-6-N', dimethyladenosine transferase
Presentation Purified
Molecular weight 37.5 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UNQ2
Purity > 90 % by SDS - PAGE
Background DIMT1 (DIM1 dimethyladenosine transferase 1 homolog) belongs to the methyltransferase superfamily. This enzyme is specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. It functions to dimethylate adjacent adenosines on the conserved hairpin loop of 18S rRNA in the 40S particle. DIMT1L is localized to the nucleolus.
Concentration 0.25mg/ml (determined by Bradford assay)
General Readings Scherl A., et al. (2002) Mol. Biol. Cell 13:4100-4109Saltman, et al. (1993) Genomics 16:726-732.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human DIMT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MMPKVKSGAI GRRRGRQEQR RELKSAGGLM FNTGIGQHIL KNPLIINSII DKAALRPTDV VLEVGPGTGN MTVKLLEKAK KVVACELDPR LVAELHKRVQ GTPVASKLQV LVGDVLKTDL PFFDTCVANL PYQISSPFVF KLLLHRPFFR CAILMFQREF ALRLVAKPGD KLYCRLSINT QLLARVDHLM KVGKNNFRPP PKVESSVVRI EPKNPPPPIN FQEWDGLVRI TFVRKNKTLS AAFKSSAVQQ LLEKNYRIHC SVHNIIIPED FSIADKIQQI LTSTGFSDKR ARSMDIDDFI RLLHGFNAEG IHFS
Molecular weight:
(334aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 2mM DTT, 200mM NaCl, 2mM EDTA
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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