Product Description for Catalase
Catalase.
Presentation: Purified
Presentation: Purified
Properties for Catalase
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CAT, Peroxisome Marker |
| Presentation | Purified |
| Molecular weight | 61.9 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 90 % by SDS - PAGE |
| Background | CAT is a key antioxidant enzyme in the bodies defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Protocols | Activity assay: 1. Prepare an assay buffer with the following concentrations: 50 mM Potassium Phosphate (pH 8.0) and 0.15 % H2O2. 2. Load 150ul assay buffer into each well. 3. Add 50ul of recombinant CAT protein with 0.25ug, 0.125ug to each well. 4. Record the decrease in A260nm for 5 minutes at 25°C using UV plate. |
| General Readings | Freitas MO, et al. (2011) J Biol Chem. 2011 Nov 25;286(47):40509-19. Epub 2011 Oct 5. |
| Storage | Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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