AR50441PU-N EMG1 / C2F (1-244, His-tag)

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0.5 mg / €820.00
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EMG1 / C2F

Product Description for EMG1 / C2F

EMG1 / C2F.
Presentation: Purified

Properties for EMG1 / C2F

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1, 18S rRNA Psi1248 methyltransferase, NEP1, Nucleolar protein EMG1 homolog, Ribosomal RNA small subunit methyltransferase NEP1, Ribosome biogenesis protein NEP1
Presentation Purified
Molecular weight 29.1 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q92979
Purity > 95 % by SDS - PAGE
Background EMG1 nucleolar protein homolog, also known as EMG1, is a 244 amino acid protein that is thought to be involved in ribosome biogenesis. Localized to the nucleolus, EMG1 participates in pre-18S rRNA processing and may play an important role in the assembly of the small ribosomal subunit, possibly controlling methylation during ribosome synthesis. A mutation in this gene has been associated with Bowen-Conradi syndrome.
Concentration 1 mg/ml (determined by Bradford assay)
General Readings Buchhaupt M., et al. (2001) Mol Genet Genomics. 276: 273-284Liu P C., et al. (2001) Mol Biol Cell. 12: 3644-3657.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human EMG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI
Molecular weight:
(267aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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