AR50408PU-S Calponin-1 (1-297, His-tag)

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0.1 mg / €320.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Calponin-1

Product Description for Calponin-1

Calponin-1.
Presentation: Purified

Properties for Calponin-1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms Basic calponin, CNN1, Calponin H1
Presentation Purified
Molecular weight 34.2 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P51911
Purity > 90 % by SDS - PAGE
Background CNN1, also known as calponin-1, belongs to the calponin family. CNN1 is thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings
  1. Maguchi M, Nishida W, Kohara K, Kuwano A, Kondo I, Hiwada K. Molecular cloning and gene mapping of human basic and acidic calponins. Biochem Biophys Res Commun. 1995 Dec 5;217(1):238-44. PubMed PMID: 8526917.
  2. Miano JM, Krahe R, Garcia E, Elliott JM, Olson EN. Expression, genomic structure and high resolution mapping to 19p13.2 of the human smooth muscle cell calponin gene. Gene. 1997 Sep 15;197(1-2):215-24. PubMed PMID: 9332369.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human CNN1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSALEH HHHHH
Molecular weight:
(305aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 30% Glycerol, 0.15M NaCl, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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