ERCC1 (1-273, His-tag) - AR50370PU-S

Product Description for ERCC1

ERCC1.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	50 µg
Synonyms	COFS4, DNA excision repair protein ERCC-1, UV20
Presentation	Purified
Molecular weight	32.5 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P07992
Purity	> 90 % by SDS - PAGE
Background	ERCC1, also known as DNA excision repair protein ERCC-1, functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4).
Concentration	0.5 mg/ml (determined by Bradford assay)
General Readings	van Duin M., et al. (1986) Cell. 44:913-923Grimwood J., et al. (2004) Nature. 428:529-535
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human ERCC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSHMDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAA PQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDV IPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTL ILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLH EPFLKVP` Molecular weight: (297aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT Purity: >90% by SDS - PAGE State: Liquid purified protein Purified