EGF (977-1029, His-tag) - AR50356PU-N

Product Description for EGF

EGF.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.1 mg
Synonyms	Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Presentation	Purified
Molecular weight	8.6 kDa
Source	E. coli
Species (Protein)	Mouse
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P01132
Purity	> 90 % by SDS - PAGE
Concentration	0.5mg/ml (determined by Bradford assay)
General Readings	Riese., et al (1998 ) Bioessays. 20: 41-48. Cohen S., (1983) Cancer. 15: 1787-1791. Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant mouse epidermal growth factor (EGF) is a 8.6 kDa globular protein containing 77 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant mouse EGF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR` Molecular weight: (77aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl Purity: >90% by SDS - PAGE State: Liquid purified protein Purified