Tumor necrosis factor (TNF-alpha) (80-235) - AR50300PU-N

Product Description for Tumor necrosis factor (TNF-alpha)

Tumor necrosis factor (TNF-alpha).
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Presentation	Purified
Molecular weight	17.4 kDa
Source	E. coli
Species (Protein)	Mouse
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P06804
Purity	> 95 % by SDS - PAGE
Background	TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia.
Concentration	1 mg/ml (determined by Bradford assay)
General Readings	Shakhov A.N., et al. (1987) Bioorg. Khim. 13:701-705Pennica D., et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82:6060-6064
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant mouse TNFa protein was expressed in E.coli and purified by using conventional chromatography. AA Sequence: `MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL` Molecular weight: (157aa), confirmed by MALDI-TOF
Format	Buffer System: Phosphate buffered saline (pH 7.4) Purity: >95% by SDS - PAGE State: Liquid purified protein Purified