MAD2L1-binding protein (1-274, His-tag) - AR50266PU-S

Product Description for MAD2L1-binding protein

MAD2L1-binding protein.
Presentation: Purified

Immunogen	Swiss Prot Num: Q15013
Purity	> 90 % by SDS - PAGE
Background	MAD2L1-binding protein, also known as MAD2L1BP, belongs to the MAD2L1BP family. This protein may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
Concentration	0.5mg/ml (determined by Bradford assay)
General Readings	Habu T, et al. (2002). EMBO J. 21(23): 6419-28.Howell BJ, et al. (2000) J Cell Biol. 18;150(6):1233-50.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human MAD2L1BP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSHMAAPEA EVLSSAAVPD LEWYEKSEET HASQIELLET SSTQEPLNAS EAFCPRDCMV PVVFPGPVSQ EGCCQFTCEL LKHIMYQRQQ LPLPYEQLKH FYRKPSPQAE EMLKKKPRAT TEVSSRKCQQ ALAELESVLS HLEDFFARTL VPRVLILLGG NALSPKEFYE LDLSLLAPYS VDQSLSTAAC LRRLFRAIFM ADAFSELQAP PLMGTVVMAQ GHRNCGEDWF RPKLNYRVPS RGHKLTVTLS CGRPSIRTTA WEDYIWFQAP VTFKGFRE` Molecular weight: (298aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl Purity: >90% by SDS - PAGE State: Liquid purified protein Purified