AR50296PU-N Alcohol dehydrogenase 5 / ADH5 (1-374, His-tag)

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0.5 mg / €820.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Alcohol dehydrogenase 5 / ADH5

Product Description for Alcohol dehydrogenase 5 / ADH5

Alcohol dehydrogenase 5 / ADH5.
Presentation: Purified

Properties for Alcohol dehydrogenase 5 / ADH5

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms Alcohol dehydrogenase class 3 chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase
Presentation Purified
Molecular weight 42.3 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P11766
Purity > 90 % by SDS - PAGE
Background ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Zhang,X. et al. (2010) Carcinogenesis 31 (12), 2118-2123Shimada,M. et al. (2010) Hum. Genet. 128 (4), 433-441
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human ADH5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMANEVI KCKAAVAWEA GKPLSIEEIE VAPPKAHEVR IKIIATAVCH TDAYTLSGAD PEGCFPVILG HEGAGIVESV GEGVTKLKAG DTVIPLYIPQ CGECKFCLNP KTNLCQKIRV TQGKGLMPDG TSRFTCKGKT ILHYMGTSTF SEYTVVADIS VAKIDPLAPL DKVCLLGCGI STGYGAAVNT AKLEPGSVCA VFGLGGVGLA VIMGCKVAGA SRIIGVDINK DKFARAKEFG ATECINPQDF SKPIQEVLIE MTDGGVDYSF ECIGNVKVMR AALEACHKGW GVSVVVGVAA SGEEIATRPF QLVTGRTWKG TAFGGWKSVE SVPKLVSEYM SKKIKVDEFV THNLSFDEIN KAFELMHSGK SIRTVVKI
Molecular weight:
(398aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH7.5) containing 20% glycerol 0.1M Nacl, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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