AR50199PU-N ATF1 (1-271, His-tag)

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0.25 mg / €1,070.00
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Quick Overview

ATF1

Product Description for ATF1

ATF1.
Presentation: Purified

Properties for ATF1

Product Category Proteins & Growth Factors
Quantity 0.25 mg
Synonyms ATF-1, Activating transcription factor 1, Cyclic AMP-dependent transcription factor ATF-1, Protein TREB36, cAMP-dependent transcription factor ATF-1
Presentation Purified
Molecular weight 31.8 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P18846
Purity > 90 % by SDS - PAGE
Background Activating transcription factor 1, also known as ATF1, is a cyclic-AMP dependent transcription factor. ATF1 is expressed in a wide variety of cell types and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factor induction of c-jun expression requires ATF1 and MEF2 sites in the c-jun promoter.
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings Lin Y S., et al. (1988) Proc Natl Acad Sci USA. 85:3396-3400.Gupta P., et al. (2002) J Biol Chem. 277:50550-50556.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human ATF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSD SIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMS VPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQ VVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKE YVKCLENRVA VLENQNKTLI EELKTLKDLY SNKSV
Molecular weight:
(295aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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