AR50095PU-N UBE2D3 (1-149, His-tag)

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0.25 mg / €820.00
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UBE2D3

Product Description for UBE2D3

UBE2D3.
Presentation: Purified

Properties for UBE2D3

Product Category Proteins & Growth Factors
Quantity 0.25 mg
Synonyms UBC5C, UBCH5C, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2 D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3
Presentation Purified
Molecular weight 19.1 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P61077
Purity > 90 % by SDS - PAGE
Background UBE2D3, also known as ubiquitin-conjugating enzyme E2 D3. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings Gonen H., et al. (1999) J. Biol. Chem. 274:14823-14830Murata S., et al. (2001) EMBO Rep. 2:1133-1138
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human UBE2D3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MLSNRKCLSK ELSDLARDPP AQCSAGPVGD DMFHWQATIM GPNDSPYQGG VFFLTIHFPT DYPFKPPKVA FTTRIYHPNI NSNGSICLDI LRSQWSPALT ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDRDKYNRIS REWTQKYAM
Molecular weight:
(169aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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