GCSH (49-173, His-tag) - AR50106PU-N

Product Description for GCSH

GCSH.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	Glycine cleavage system H protein
Presentation	Purified
Molecular weight	16.4 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P23434
Purity	> 95 % by SDS - PAGE
Background	The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
Concentration	1 mg/ml (determined by Bradford)
General Readings	Kure S., et al. (2001) J Hum Genet. 46(7):378-84.Kovata H., et al. (1991) Am J Hum Genet. 48(2):351-61.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human GCSH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMSVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE` Molecular weight: (149aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl. Purity: >95% by SDS - PAGE State: Liquid purified protein Purified