AR50048PU-S BLK (1-505, His-tag)

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50 µg / €400.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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BLK

Product Description for BLK

BLK.
Presentation: Purified

Properties for BLK

Product Category Proteins & Growth Factors
Quantity 50 µg
Synonyms B lymphocyte kinase, Tyrosine-protein kinase BLK, p55-BLK, p55BLK
Presentation Purified
Molecular weight 59.8 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P51451
Purity > 80 % by SDS - PAGE
Background BLK, also known as B lymphoid kinase, is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. This protein is expressed specifically in the B cell lineage and plays a role in the signaltransduction pathway that is restricted to B lymphoid cells.
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings Dymecki. et al. (1990) Science. 247:332-6.Tatosyan, A.G. et al. (2000) Biochemistry. 65:49-58
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human BLK protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP
Molecular weight:
(525aa) and 50-70kDa (SDS-PAGE under reducing conditions)
Format
Buffer System:
20mM Tris-HCl buffer (pH7.5) containing 50% glycerol, 0.25M NaCl, 5mM DTT, 0.1mM PMSF, 0.1M EDTA
Purity:
>80% by SDS - PAGE
State:
Liquid purified protein
Purified
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