AR50109PU-N Isocitrate dehydrogenase / IDH (1-414, His-tag)

Swiss Prot Num:

O75874

2. Add 50ul of recombinant protein IDH1 with 1 ug, 2 ug, and 5 ug in assay buffer.

3. Mix by inversion and load 200 ul of reaction mix in to a plate well. chloride.

4. Record the increase in A340 nm for 5 minutes at 25C.

Description:

Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

AA Sequence:

MGSSHHHHHH SSGLVPRGSH MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKL

Molecular weight:

(434aa), confirmed by MALDI-TOF

Specific activity:

> 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.

Buffer System:

20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT,0.1mM PMSF

Purity:

>95% by SDS - PAGE

State:

Liquid purified protein
Purified