Product Description for FKBP1B / FKBP9
FKBP1B / FKBP9.
Presentation: Purified
Presentation: Purified
Properties for FKBP1B / FKBP9
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | FK506-binding protein 1B, FKBP12.6, FKBP1L, Immunophilin FKBP12.6, OTK4, PPIase FKBP1B, Peptidyl-prolyl cis-trans isomerase FKBP1B, Rotamase, h-FKBP-12 |
| Presentation | Purified |
| Molecular weight | 14.2 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 90 % by SDS - PAGE |
| Background | FKBP1B, also known as peptidyl-prolyl cis-trans isomerase FKBP1B, is a member of the immunophilin protein family which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus). It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Protocols | 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant FKBP1B protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5. Record the increase in A405 nm for 30 minutes at 25C. |
| General Readings | Arakawa H., et al. (1994) Biochem. Biophys. Res. 200:836-843Deivanayagam C.C., et al. (2000) Acta Crystallogr. D 56:266-271 |
| Storage | Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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