TDP1 (1-298, His-tag) - AR50774PU-S

Product Description for TDP1

TDP1.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.1 mg
Synonyms	FLJ11090, SCAN1, TDP-1, Tyr-DNA phosphodiesterase 1, Tyrosyl DNA phosphodiesterase 1, Tyrosyl-DNA phosphodiesterase 1
Presentation	Purified
Molecular weight	35.8 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: Q9BRS7
Purity	> 90 % by SDS - PAGE
Background	Tyrosyl-DNA phosphodiesterase 1, also known as TDP1, is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks.
Concentration	1 mg/ml (determined by Bradford assay)
General Readings	Heilig R., et al. (2003) Nature. 421:601-607The MGC Project Team. (2004) Genome Res. 14:2121-2127
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human TDP1, fused to His-tag at N-terminus, was expressed in E.coli. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MSQEGDYGRW TISSSDESEE EKPKPDKPST SSLLCARQGA ANEPRYTCSE AQKAAHKRKI SPVKFSNTDS VLPPKRQKSG SQEDLGWCLS SSDDELQPEM PQKQAEKVVI KKEKDISAPN DGTAQRTENH GAPACHRLKE EEDEYETSGE GQDIWDMLDK GNPFQFYLTR VSGVKPKYNS GALHIKDILS PLFGTLVSSA QFNYCFDVDW LVKQYPPEFR KKPILLVHGD KREAKAHLHA QAKPYENISL CQAKLDIAFG THHTKMMLLL YEEGLRVVIH TSNLIHADWH QKTQGTHL` Molecular weight: (318aa)
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol Purity: >90% by SDS - PAGE State: Liquid purified protein Purified