Histone H3.3 (1-136, His-tag) - AR50907PU-N

Product Description for Histone H3.3

Histone H3.3.
Presentation: Purified

Immunogen	Swiss Prot Num: P84243
Purity	> 90 % by SDS - PAGE
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A is a replication-independent member of the histone H3 family.
Concentration	1 mg/ml (determined by Bradford assay)
General Readings	Tagami H., et al. (2004) Cell. 116:51-61Daury L N., et al. (2006) EMBO Rep. 7:66-71
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human H3F3A protein, fused to His-tag at N-terminus, was expressed in E.coli. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA` Molecular weight: > 90% by SDS - PAGE
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol Purity: >90% by SDS - PAGE State: Liquid purified protein Purified