U2AF1 / U2AF35 (1-240, His-tag) - AR50919PU-N

Product Description for U2AF1 / U2AF35

U2AF1 / U2AF35.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AFBP
Presentation	Purified
Molecular weight	30.3 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: Q01081
Purity	> 90 % by SDS - PAGE
Background	U2AF1 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This protein is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Concentration	1 mg/ml (determined by Bradford assay)
General Readings	Thol,F., et al. (2012) Blood 119 (15), 3578-3584Makishima,H., et al. (2012) Blood 119 (14), 3203-3210
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human U2AF1 protein, fused to His-tag at N-terminus, was expressed in E.coli. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF` Molecular weight: (263aa)
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol Purity: >90% by SDS - PAGE State: Liquid purified protein Purified