discontinued

SM018FS CD19 antibody

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Rat anti Mouse CD19 6D5

SM018FS

Product Description for CD19

Rat anti Mouse CD19 6D5.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD19

Product Category Primary Antibodies
Quantity 50 µg
Synonyms B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
Presentation FITC
Reactivity Ms
Applications F
Clonality Monoclonal
Clone 6D5
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P25918
Immunogen:
Human K562 cell line transfected with murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
GeneID:
12478
Application Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Background CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.
Concentration 0.1 mg/ml
General Readings
  1. Krop, I. et al. (1996) Antibody to CD19 suppresses self-renewal of B-1 lymphocytes. Eur. J. Immunol. 26: 238.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
State:
Liquid purified IgG
Specificity
Specificity:
This antibody recognises the CD19 cell surface antigen, a 95kD glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.
Species:
Mouse.
Gene ID 12478

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