AR51164PU-N DBR1 (228-544, His-tag)

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0.25 mg / €1,070.00
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Quick Overview

DBR1

Product Description for DBR1

DBR1.
Presentation: Purified

Properties for DBR1

Product Category Proteins & Growth Factors
Quantity 0.25 mg
Synonyms DBR-1, Debranching enzyme homolog 1, Lariat debranching enzyme
Presentation Purified
Molecular weight 38 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UK59
Purity > 90 % by SDS - PAGE
Background DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Ye Y, De Leon J, et al. (2005). Retrovirology. 2:63.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description
Description:
Recombinant human DBR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA
Molecular weight:
(340aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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