discontinued

SM1198F CD89 / FCAR antibody

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Quick Overview

Mouse anti Human CD89 / FCAR MIP8a

SM1198F

Product Description for CD89 / FCAR

Mouse anti Human CD89 / FCAR MIP8a.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD89 / FCAR

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms IgA Fc receptor, Immunoglobulin alpha Fc receptor
Presentation FITC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone MIP8a
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P24071
Immunogen:
Recombinant soluble Human FcaR.
GeneID:
2204
Isotype control SM10F (for use in human samples)
Application Flow Cytometry: Use 10 µl of neat diluted antibody to label 106 cells or 100 µl. 
The preservative free format of this antibody is recommended for Functional Studies.
Background CD89 is a 50-75kD cell surface glycoprotein that is also known as the IgA receptor (Fc alpha R). CD89 is expressed by peripheral blood monocytes and neutrophils.
Concentration 0.1 mg/ml
General Readings
  1. Zhang W, Bi B, Oldroyd RG, Lachmann PJ. Neutrophil lactoferrin release induced by IgA immune complexes differed from that induced by cross-linking of fcalpha receptors (FcalphaR) with a monoclonal antibody, MIP8a. Clin Exp Immunol. 2000 Jul;121(1):106-11. PubMed PMID: 10886246. (Free PMC Article available, 6 images available)
  2. Lu J, Marjon KD, Marnell LL, Wang R, Mold C, Du Clos TW, et al. Recognition and functional activation of the human IgA receptor (FcalphaRI) by C-reactive protein. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4974-9. doi: 10.1073/pnas.1018369108. Epub 2011 Mar 7. PubMed PMID: 21383176. (Free PMC Article available, 6 images available)
  3. Duc M, Johansen FE, Corthésy B. Antigen binding to secretory immunoglobulin A results in decreased sensitivity to intestinal proteases and increased binding to cellular Fc receptors. J Biol Chem. 2010 Jan 8;285(2):953-60. doi: 10.1074/jbc.M109.059220. Epub 2009 Nov 12. PubMed PMID: 19910466. (Free PMC Article available, 5 images available)
  4. Wu J, Ji C, Xie F, Langefeld CD, Qian K, Gibson AW, et al. FcalphaRI (CD89) alleles determine the proinflammatory potential of serum IgA. J Immunol. 2007 Mar 15;178(6):3973-82. PubMed PMID: 17339498.
  5. Hamre R, Farstad IN, Brandtzaeg P, Morton HC. Expression and modulation of the human immunoglobulin A Fc receptor (CD89) and the FcR gamma chain on myeloid cells in blood and tissue. Scand J Immunol. 2003 Jun;57(6):506-16. PubMed PMID: 12791088.
  6. Qian K, Xie F, Gibson AW, Edberg JC, Kimberly RP, Wu J. Functional expression of IgA receptor FcalphaRI on human platelets. J Leukoc Biol. 2008 Dec;84(6):1492-500. doi: 10.1189/jlb.0508327. Epub 2008 Sep 10. PubMed PMID: 18784345. (Free PMC Article available, 6 images available)
  7. Van Egmond, M. (2011) Method for the treatment or prophylaxis of chronic inflammatory diseases. European Patent Application No: 12/736963
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction
Specificity
Specificity:
This antibody recognises the CD89 cell surface antigen, a 50-75kD cell surface glycoprotein that is also known as the IgA receptor (Fc alpha R). 
Clone MIP8a blocks binding of IgA to the Fc alpha R, and also inhibits neutrophil phagocytosis of IgA complexes.
Species:
Human.
Gene ID 2204

Accessory Products

Proteins and/or Positive Controls

Proteins for CD89 / FCAR (6 products)

Catalog No. Species Pres. Purity   Source  

CD89 / FCAR (transcript variant 1)

CD89 / FCAR Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD89 / FCAR (transcript variant 1)

CD89 / FCAR Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

CD89 / FCAR

CD89 / FCAR Human
  Abnova Taiwan Corp.

CD89 / FCAR

CD89 / FCAR Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD89 / FCAR

CD89 / FCAR Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD89 / FCAR

CD89 / FCAR Human
  Abnova Taiwan Corp.

Positive controls for CD89 / FCAR (7 products)

Catalog No. Species Pres. Purity   Source  

CD89 Lysate

Western Blot: CD89 Lysate [NBL1-10649] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for FCAR
  Novus Biologicals Inc.

FCAR 293T Cell Transient Overexpression Lysate(Denatured)

FCAR 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

FCAR overexpression lysate

FCAR overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

FCAR overexpression lysate

FCAR overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

FCAR overexpression lysate

FCAR overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

FCAR overexpression lysate

FCAR overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.

FCAR overexpression lysate

FCAR overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.
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