AP32827AF-N Lysozyme C antibody

Swiss Prot Num:

P00698

Immunogen:

Highly purified lysozyme is isolated from pooled milk.Freund's complete adjuvant is used in the first step of the immunization procedure.

GeneID:

396218

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Purification:

Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE-chromatography

Buffer System:

PBS, pH 7.2

Preservatives:

None

Stabilizers:

None

State:

Lyophilized Purified hyperimmune IgG fraction
Azide Free

Reconstitution:

Restore by by adding 1 ml sterile di stilled water

Species reactivity (tested):

Human.

Specificity:

The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum.
Cross-reactivity: The antiserum does not cross-react with any other component of human plasma.
Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.