TP53 / p53 pSer392 - SM2157P | acris-antibodies.com

Product Description for TP53 / p53

Mouse anti Human TP53 / p53 FP3-2.
Properties: pSer392
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for TP53 / p53

Product Category	Primary Antibodies
Quantity	0.1 mg
Synonyms	Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Presentation	Purified
Reactivity	Hu
Applications	IP, P, WB
Clonality	Monoclonal
Clone	FP3-2
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P04637 Immunogen: Synthetic peptide RKKLMFKTEGPDS[P]D GeneID: 7157 Remarks: Spleen cells from immunised BALB/c mice were fused with cells of a mouse SP-2 myeloma cell line
Property	pSer392
Isotype control	AM03095PU-N, SM10P (for use in human samples)
Application	Western Blot: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are *not* recommended as casein is a phosphoprotein and its use can result in high background. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Colon or breast carcinoma.
Background	Ser392 phosphorylation of p53 has been shown to occur following DNA damage. Phosphorylation of p53 at this site may be mediated the casein kinase 2 (CK2), and also be other phosphorylation mechanisms.
Concentration	1.0 mg/ml
General Readings	Blaydes JP, Hupp TR. DNA damage triggers DRB-resistant phosphorylation of human p53 at the CK2 site. Oncogene. 1998 Aug 27;17(8):1045-52. PubMed PMID: 9747884.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein A Buffer System: PBS Preservatives: 0.09% Sodium Azide State: Liquid purified IgG fraction Purified
Specificity	Specificity: This antibody recognises p53, specifically binding only to p53 phosphorylated at the Ser392 residue. In immunohistology this antibody generally stains 5-10% of cells labelled with antibodies specific for non-phosphorylated p53. Species: Human.
Gene ID	7157

Accessory Products

Proteins and/or Positive Controls

Proteins for TP53 / p53 (23 products)

Catalog No.	Species	Pres.	Purity	Source
TP300003	TP53 / p53 (transcript variant 1)
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP325502	TP53 / p53 (transcript variant 3)
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP325604	TP53 / p53 (transcript variant 2)
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP701001	TP53 / p53 (transcript variant 1)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP710022	TP53 / p53 (full length, C-term DDK tag, transcript variant 1)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	SF9 cells	20 µg / €411.00
					OriGene Technologies, Inc.
AR10316PU-L	TP53 / p53
	Human	Purified	> 95.0% as determined by SDS-PAGE	E. coli
					OriGene Technologies GmbH
AR10316PU-N	TP53 / p53
	Human	Purified	> 95.0% as determined by SDS-PAGE	E. coli
					OriGene Technologies GmbH
AR10316PU-S	TP53 / p53
	Human	Purified	> 95.0% as determined by SDS-PAGE	E. coli
					OriGene Technologies GmbH
PA1366	TP53 / p53 (Mutant)
	Human	Purified	> 90 %	E. coli
					OriGene Technologies GmbH
PA1366X	TP53 / p53 (Mutant)
	Human	Purified	> 90 %	E. coli
					OriGene Technologies GmbH
PA1366XC	TP53 / p53 (Mutant)
	Human	Purified	> 90 %	E. coli
					OriGene Technologies GmbH
PA1368	TP53 / p53
	Human	Purified	> 95 % pure as determined by SDS-PAGE.	E. coli
					OriGene Technologies GmbH
PA1368X	TP53 / p53
	Human	Purified	> 95 % pure as determined by SDS-PAGE.	E. coli
					OriGene Technologies GmbH
PA1368XC	TP53 / p53
	Human	Purified	> 95 % pure as determined by SDS-PAGE.	E. coli
					OriGene Technologies GmbH
H00007157-P01	TP53 / p53
	Human
					Abnova Taiwan Corp.
H00007157-P01-10	TP53 / p53
	Human			in vitro transl.
					Abnova Taiwan Corp.
H00007157-P01-25	TP53 / p53
	Human			in vitro transl.
					Abnova Taiwan Corp.
H00007157-P02	TP53 / p53
	Human	Purified
					Abnova Taiwan Corp.
H00007157-P02-10	TP53 / p53
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00007157-P02-25	TP53 / p53
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00007157-Q01-10	TP53 / p53
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00007157-Q01-25	TP53 / p53
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P5316-R-20	TP53 / p53 (Wild type)
	Human	Purified	> 95 %	E. coli
					Alpha Diagnostic Intl. Inc.

Positive controls for TP53 / p53 (3 products)

Catalog No.	Species
NBL1-17203	p53 Lysate

		Novus Biologicals Inc.
H00007157-T01	TP53 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.
H00007157-T02	TP53 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.

Recommended Secondary Antibodies for TP53 / p53 (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

Click here to see all secondary antibodies for TP53 / p53

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products

SM2157P TP53 / p53 pSer392 antibody

Quick Overview

Product Description for TP53 / p53

Properties for TP53 / p53

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for TP53 / p53 (23 products)

Positive controls for TP53 / p53 (3 products)

Recommended Secondary Antibodies for TP53 / p53 (11 products)

Service