AR51384PU-N Heparanase / HPSE (36-543, His-tag)

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0.5 mg / €820.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Heparanase / HPSE

Product Description for Heparanase / HPSE

Heparanase / HPSE.
Presentation: Purified

Properties for Heparanase / HPSE

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms Endo-Glucoronidase, HEP, HPA, HPA1, HPR1, HPSE1, HSE1, Heparanase-1
Presentation Purified
Molecular weight 60.0 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y251
Purity > 85 % pure by SDS-PAGE
Background Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. HPSE is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings
  1. Okada Y, Yamada S, Toyoshima M, Dong J, Nakajima M, Sugahara K. Structural recognition by recombinant human heparanase that plays critical roles in tumor metastasis. Hierarchical sulfate groups with different effects and the essential target disulfated trisaccharide sequence. J Biol Chem. 2002 Nov 8;277(45):42488-95. Epub 2002 Sep 3. PubMed PMID: 12213822.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human HPSE protein, fused to His-tag at N-terminus, was expressed in E.coli.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSQDVVDLD FFTQEPLHLV SPSFLSVTID ANLATDPRFL ILLGSPKLRT LARGLSPAYL RFGGTKTDFL IFDPKKESTF EERSYWQSQV NQDICKYGSI PPDVEEKLRL EWPYQEQLLL REHYQKKFKN STYSRSSVDV LYTFANCSGL DLIFGLNALL RTADLQWNSS NAQLLLDYCS SKGYNISWEL GNEPNSFLKK ADIFINGSQL GEDFIQLHKL LRKSTFKNAK LYGPDVGQPR RKTAKMLKSF LKAGGEVIDS VTWHHYYLNG RTATKEDFLN PDVLDIFISS VQKVFQVVES TRPGKKVWLG ETSSAYGGGA PLLSDTFAAG FMWLDKLGLS ARMGIEVVMR QVFFGAGNYH LVDENFDPLP DYWLSLLFKK LVGTKVLMAS VQGSKRRKLR VYLHCTNTDN PRYKEGDLTL YAINLHNVTK YLRLPYPFSN KQVDKYLLRP LGPHGLLSKS VQLNGLTLKM VDDQTLPPLM EKPLRPGSSL GLPAFSYSFF VIRNAKVAAC I
Molecular weight:
(531 aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
Purity:
>85% pure by SDS-PAGE
State:
Liquid purified protein
Purified
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