discontinued

SP1232P DFFA / ICAD (N-term) antibody

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Rabbit anti Mouse DFFA / ICAD

SP1232P

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  • SP1232P

Product Description for DFFA / ICAD

Rabbit anti Mouse DFFA / ICAD.
Properties: (N-term)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for DFFA / ICAD

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Presentation Purified
Reactivity Ms
Applications P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O54786
Immunogen:
Synthetic peptide corresponding to amino acids 2-21 of Mouse ICAD
GeneID:
13347
Property N-term
Application Western blot: 1/1000. This antibody recognises a 45kDa band. 
Immunohistochemistry on Paraffin Sections: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Background ICAD (Inhibitor of Caspase-Activated Dnase) is the murine homologue of human DFF-45. ICAD is cleaved by Caspase-3, releasing CAD which eventually causes degradation of DNA in the nucleus.
Concentration 1.0 mg/ml
General Readings
  1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature. 1998 Jan 1;391(6662):43-50. PubMed PMID: 9422506.
  2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature. 1998 Jan 1;391(6662):96-9. PubMed PMID: 9422513.
  3. Wyllie, A. (1998) Apoptosis: An endonuclease at last. Nature 391: 20-21.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Afinity Chromatography
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody reacts to ICAD at C-term.
Species:
Mouse.

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