Prothrombin (F2) (328-622, His-tag) - AR51483PU-S

Product Description for Prothrombin (F2)

Prothrombin (F2).
Presentation: Purified

Immunogen	Swiss Prot Num: P00734
Purity	> 80 % by SDS - PAGE
Background	Coagulation factor II, also known as F2, is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Royle NJ, Irwin DM, Koschinsky ML., et al. (1987) Somat. Cell Mol. Genet. 13 (3): 285–92
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human F2 protein was expressed in E.coli. AA Sequence: `MTFGSGEADC GLRPLFEKKS LEDKTERELL ESYIDGRIVE GSDAEIGMSP WQVMLFRKSP QELLCGASLI SDRWVLTAAH CLLYPPWDKN FTENDLLVRI GKHSRTRYER NIEKISMLEK IYIHPRYNWR ENLDRDIALM KLKKPVAFSD YIHPVCLPDR ETAASLLQAG YKGRVTGWGN LKETWTANVG KGQPSVLQVV NLPIVERPVC KDSTRIRITD NMFCAGYKPD EGKRGDACEG DSGGPFVMKS PFNNRWYQMG IVSWGEGCDR DGKYGFYTHV FRLKKWIQKV IDQFGE` Molecular weight: (296aa)
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol. Purity: >80% by SDS - PAGE State: Liquid purified protein Purified