AM33032PU-N CD36 antibody

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0.1 mg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD36 185-1G2

AM33032PU-N

Product Description for CD36

Mouse anti Human CD36 185-1G2.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for CD36

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Fatty acid translocase, GP3B, GP4, Glycoprotein IIIb, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein 4, Platelet glycoprotein IV, Thrombospondin receptor
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF
Clonality Monoclonal
Clone 185-1G2
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P16671
Immunogen:
Derived by fusion of NS1 mouse myeloma cells with spleen cells from BALB/c mice immunized with stimulated Human leucocytes.
GeneID:
948
Isotype control AM03096PU-N
Application Flow Cytometry: 1/100-1/200. 
The antibody 185-1G2 can be used for CD36 cell identification, quantification and isolation using flow cytometric methods.
Immunocytochemistry. 
Immunohistochemistry on Frozen Sections. 
Functional Assay:
The antibody has also been reported to bind to a site of CD36 that inhibits CD36 function. Please request a Sodium Azide Free antibody format.
Background The CD36 molecule is an 88kDa transmembrane glycoprotein, which is a member of the class B scavenger receptor family of proteins. Alternative names for CD36 include fatty acid translocase (FAT), platelet glycoprotein 4, platelet glycoprotein IIIb and thrombospondin receptor. CD36 is a pattern recognition receptor that is expressed on a wide variety of cells including monocytes, macrophages, platelets, adipocytes, erythrocyte precursors, activated keratinocytes and some endothelial and epithelial cells. Studies suggest that CD36 has multiple cellular functions and binds to a diverse array of ligands including thrombospondin, modified low-density lipoproteins (LDL), anionic phospholipids and long chain fatty acids.
As a scavenger receptor, CD36 plays a key role in mediating the uptake of oxidized LDL by macrophages, the formation of foam cells and the development of atherosclerosis. CD36 is also reported to function as negative regulator of angiogenesis, as a cell adhesion molecule involved in platelet adhesion and aggregation, and as a scavenger receptor in the clearance of apoptotic cells and photoreceptor rod outer segments.  Furthermore, CD36 is reported to bind toPlasmodium falciparum infected erythrocytes and may play an important role in the pathogenesis of malaria and sickle cell disease.
Concentration 1.0 mg/ml
General Readings
  1. Howard LM, Puente Navazo MD, McGregor JL. Identification of CD36-ractive antibodies that bind an epitope encompassing amino acid residues 155-183. In Leucocyte typing VI. Sixth International workshop and conference. Kishimoto T, Kikutani H, von dem Borne AEG.Kr, Goyert SA, Mason DY, Miyasaka M, Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K, Zola H. Eds. Kobe, Japan, Garland. 1996. p637-638.
  2. Englyst NA, Taube JM, Aitman TJ, Baglin TP, Byrne CD. A novel role for CD36 in VLDL-enhanced platelet activation. Diabetes. 2003 May;52(5):1248-55. PubMed PMID: 12716760.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Lyophilized purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The Monoclonal antibody 185-1G2 recognizes an epitope between amino acids 155–183 on the CD36 molecule.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD36 (13 products)

Catalog No. Species Pres. Purity   Source  

CD36 (transcript variant 3)

CD36 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD36 (transcript variant 1)

CD36 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD36 (transcript variant 4)

CD36 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD36 (transcript variant 5)

CD36 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD36 (full length, C-term polyhistidine tag)

CD36 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

CD36

CD36 Human
  Abnova Taiwan Corp.

CD36

CD36 Human in vitro transl.
  Abnova Taiwan Corp.

CD36

CD36 Human in vitro transl.
  Abnova Taiwan Corp.

CD36

CD36 Human Purified
  Abnova Taiwan Corp.

CD36

CD36 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD36

CD36 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD36

CD36 Human
  Abnova Taiwan Corp.

CD36

CD36 Human
  Abnova Taiwan Corp.

Positive controls for CD36 (2 products)

Catalog No. Species Pres. Purity   Source  

CD36 Lysate

Western Blot: CD36 Lysate [NBL1-08939] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD36
  Novus Biologicals Inc.

CD36 Lysate

Western Blot: CD36 Lysate [NBL1-08940] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD36
  Novus Biologicals Inc.
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