AM33033PU-N CD50 / ICAM3 antibody

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0.1 mg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human CD50 / ICAM3 MA4

AM33033PU-N

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  • AM33033PU-N
  • AM33033PU-N

Product Description for CD50 / ICAM3

Mouse anti Human CD50 / ICAM3 MA4.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Frozen Sections, Flow Cytometry.

Properties for CD50 / ICAM3

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CDw50, ICAM-3, ICAM-R, Intercellular adhesion molecule 3
Presentation Purified
Reactivity Hu
Applications C, E, F, ICC/IF, IP, WB
Clonality Monoclonal
Clone MA4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P32942
Immunogen:
Derived by fusion of mouse Ag8.653 cells with spleen cells from a BALB/c mouse immunized with an ICAM-3/HEK transfectant.
GeneID:
3385
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA. 
Western Blot.
Immunoprecipitation.
Flow Cytometry. 
Immunocytochemistry.
Immunohistochemistry on Frozen Sections. 
Recommended Dilutions: 1/100–1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for Immunoblotting applications.
Background Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a type I transmembrane glycoprotein, which normally contain 2-9 immunoglobulin-like C2-type domains, and binds to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 has previously been implicated in apoptotic cell clearance, although its precise role in the clearance process is ill defined.
Concentration 1.0 mg/ml
General Readings
  1. Torr EE, Gardner DH, Thomas L, Goodall DM, Bielemeier A, Willetts R, et al. Apoptotic cell-derived ICAM-3 promotes both macrophage chemoattraction to and tethering of apoptotic cells. Cell Death Differ. 2012 Apr;19(4):671-9. doi: 10.1038/cdd.2011.167. Epub 2011 Nov 25. PubMed PMID: 22117198. (Free PMC Article available, 6 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This MA4 antibody recognizes a linear epitope in domains 1 and 2 of ICAM-3. MA4 robustly inhibits apoptotic cell interaction with phagocytes.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD50 / ICAM3 (4 products)

Catalog No. Species Pres. Purity   Source  

CD50 / ICAM3

CD50 / ICAM3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD50 / ICAM3

CD50 / ICAM3 Human
  Abnova Taiwan Corp.

CD50 / ICAM3

CD50 / ICAM3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD50 / ICAM3

CD50 / ICAM3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.
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