Product Description for Coagulation factor XIIIa (F13A1)
Rabbit anti Human Coagulation factor XIIIa (F13A1).
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.
Properties for Coagulation factor XIIIa (F13A1)
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 1 ml |
| Synonyms | Coagulation factor XIII A chain, F13A, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain |
| Presentation | Serum |
| Reactivity | Hu |
| Applications | ID, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Add. information | Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application | Immunoprecipitation. Can be used in precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FXIII. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Increase in plasma FXIII has been observed in type IV hyperlipoproteinaemia and in combined hyperlipodaemia. Congenital and acquired FXIII deficiencies have also been reported. In some cases of myeloma, inhibition of FXIII activity by the paraproteins may occur. Directions for use: In immunoelectrophoresis in agarose-plates use 2 µl human plasma or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion the amount of antiserum required in the agarose gel is usually between 1 and 2% depending on the test arrangement. Precipitin Titre: not less than 1:32 when tested against appropriate concentrations of the antigen in agar-block immunodiffusion titration. The titre is further assayed by quantitative precipitin analysis incubating serial dilutions of the antiserum with normal plasma and measuring the residual antigen. The amount of Factor XIII precipitated by 1 ml antiserum is about 10 U. |
| Background | Plasma FXIII is an active transglutaminase until it is activated (FXIIIa). It consists of two A subunits joined as a dimer, connected to two B subunits (A2B2). Each A subunit has a molecular weight of 75,000 and each B subunit of 80,000, making an aggregate molecular weight of the whole molecule of 320,000. Normal plasma contains about 20mg/ml. Platelet FXIII consists of only A subunits synthesized in the megakaryocytes. It accounts for about 50% of the total blood FXIII activity with a linear relationship between FXIII level and platelet counts. RAHu/FXIII-A reacts with both plasma and platelet FXIII. Antiserum raised against the B subunit (RAHu/FXIII-S) reacts exclusively with native and activated FXIIIa in plasma. FXIIIa results from activation of FXIII by thrombin after the release of fibrinopeptides A from fibrinogen. |
| Concentration | Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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Accessory Products
Proteins and/or Positive Controls
Proteins for Coagulation factor XIIIa (F13A1) (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP306464 |
Coagulation factor XIIIa (F13A1) |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| AR05011PU-N |
Coagulation factor XIIIa (F13A1) |
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|
Human | Purified | |||||
| OriGene Technologies GmbH | |||||||
| H00002162-P01-10 |
Coagulation factor XIIIa (F13A1) |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00002162-P01-25 |
Coagulation factor XIIIa (F13A1) |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Coagulation factor XIIIa (F13A1) (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00002162-T01 |
F13A1 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| LY400044 |
F13A1 overexpression lysate |
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|
0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | |||||||
| NBL1-10406 |
Factor XIIIa Lysate |
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![Western Blot: Factor XIIIa Lysate [NBL1-10406] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for F13A1.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Factor%20XIIIa%20Lysate-Western%20Blot-NBL1-10406-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Coagulation factor XIIIa (F13A1) (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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