AP33076SU-N SERPIND1 antibody

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1 ml / €430.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human SERPIND1

AP33076SU-N

Product Description for SERPIND1

Rabbit anti Human SERPIND1.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for SERPIND1

Product Category Primary Antibodies
Quantity 1 ml
Synonyms HC-II, HCF2, HLS2, Heparin cofactor 2, Protease inhibitor leuserpin-2, Serpin D1
Presentation Serum
Reactivity Hu
Applications ID, IP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05546
Immunogen:
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
3053
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Immunoprecipitation.
Can be used in precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of heparin cofactor II in human plasma or other body fluids or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
Directions for use: In immunoelectrophoresis in agarose-plates use 2 µl human plasma or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion the amount of antiserum required in the agarose gel is usually between 1 and 2% depending on the test arrangement.
Antibody Titre: Measured by quantitative precipitin analysis. The amount of heparin cofactor II precipitated by 1 ml antiserum is approximately 90 µg/ml.
Precipitin Titre: 1/64 when tested against pooled normal human plasma in agar-block immunodiffusion titration.
Background Heparin cofactor II is a glycoprotein synthesized in the liver and has a molecular weight of 65,000. It is a plasma serine inhibitor of thrombin. Antithrombin III is more slowly inhibited. A hereditary form of inactive heparin cofactor II has been described. Reduced plasma values have been observed in disseminated intravascular coagulopathy and in lever disease.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
None
State:
Delipidated, heat inactivated, lyophilized, stable whole antiserum
Serum
Reconstitution:
Restore 1 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is restricted to heparin cofactor II. In bidimensional electrophoresis against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified immunogen. No reaction is obtained with any other plasma protein component or serum.
Cross-reactivity: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for SERPIND1 (4 products)

Catalog No. Species Pres. Purity   Source  

SERPIND1 (58-499, His-tag)

SERPIND1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

SERPIND1 (58-499, His-tag)

SERPIND1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

SERPIND1

SERPIND1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

SERPIND1

SERPIND1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for SERPIND1 (1 products)

Catalog No. Species Pres. Purity   Source  

SERPIND1 293T Cell Transient Overexpression Lysate(Denatured)

SERPIND1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
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