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BM5042 Cytokeratin 5 (+ KRT8) antibody

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50 µg / €310.00
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Quick Overview

Mouse anti Amphibian, Bovine, Human, Hydra, Mouse, Porcine Cytokeratin 5 C22 (Ks 5+8.22)

BM5042

Product Description for Cytokeratin 5

Mouse anti Amphibian, Bovine, Human, Hydra, Mouse, Porcine Cytokeratin 5 C22 (Ks 5+8.22).
Properties: (+ KRT8)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 5

Product Category Primary Antibodies
Quantity 50 µg
Synonyms 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5
Presentation Purified
Reactivity Amph, Bov, Hu, Hydra, Ms, Por
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone C22 (Ks 5+8.22)
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13647
Immunogen:
Human keratin K8, purified from SDS PAGE gel.
GeneID:
3852
Property + KRT8
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot.
Immunocytology.
Immunohistochemistry on Frozen Tissues: 1/10.
Incubation time: 1 h at RT.
Immunohistochemistry on Paraffin-Embedded Tissues: 1/10. 
Protease pretreatment is required prior to antibody application.
Incubation time: Overnight at 2-8°C.
Background Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
General Readings
  1. Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. PubMed PMID: 1716305.
  2. Heid H, Vojtesek B, Bruder G, Kaufman M, Staskova Z, Kovarik J, Bartek J, Franke WW: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991)
  3. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379.
  4. Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane EB. Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry. 2004 Feb 10;43(5):1283-95. PubMed PMID: 14756564.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
Final solution contains 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer in PBS buffer, pH 7.4
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in 1 ml distilled water.
Specificity
Specificity:
Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia.
Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells.
Epitope has been mapped to aa 353-367 on alpha helical rod domain (see Waseem et al., 2004).
Reactivities on cultured cell lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.
Epitope has been mapped to aa 353-367 on alpha helical rod domain (See Waseem et al. 2004).
Species:
Human, Bovine, Murine, Amphibia, Pig and Hydra.
Gene ID 3852

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 5 (4 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 5 (1-590, His-tag)
Cytokeratin 5 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

Cytokeratin 5 (1-590, His-tag)
Cytokeratin 5 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

Cytokeratin 5
Cytokeratin 5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Cytokeratin 5
Cytokeratin 5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 5 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 5 Lysate
Western Blot: Cytokeratin 5 Lysate [NBL1-12393] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT5.
  Novus Biologicals Inc.

KRT5 overexpression lysate
KRT5 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

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Click here to see all secondary antibodies for Cytokeratin 5
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