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DDX0209P-50 CD209 / DC-SIGN antibody

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Mouse anti Human CD209 / DC-SIGN 111H2.02

DDX0209P-50

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Product Description for CD209 / DC-SIGN

Mouse anti Human CD209 / DC-SIGN 111H2.02.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections, Flow Cytometry.

Properties for CD209 / DC-SIGN

Product Category Primary Antibodies
Quantity 50 µg
Synonyms C-type lectin domain family 4 member L, CLEC4L, DC-SIGN1, DCSIGN, DCSIGN1, Dendritic Cell Marker, Dendritic cell-specific ICAM-3-grabbing non-integrin 1
Presentation Purified
Reactivity Hu
Applications C, F, P
Clonality Monoclonal
Clone 111H2.02
Host Mouse
Isotype IgG2b
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NNX6
Immunogen:
HeLa cells stably transfected-with human DC-SIGN.
GeneID:
30835
Isotype control AM03110PU-N, SM12P
Application DDX0209P-50 / DDX0209P-100 purified: Surface flow cytometry, IHC Formol- paraffin, Bouin paraffin, Histowax.
DDX0209A488-50 / DDX0209A488-100 Alexa-Fluor®488: Surface Flow cytometry.
DDX0209A546-50 / DDX0209A546-100 Alexa-Fluor®647: IF. 
DDX0209A647-50 / DDX0209A647-100 Alexa-Fluor®647: Surface Flow cytometry. 
DDX0209B-50 DDX0209B-100 Biotin (on request): Surface Flow cytometry, IHC. 
Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”)/CD209 and liver/lymph node-specific ICAM-3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120 (Soilleux EJ. 2003, Clinical Science 104, 437-; Dakappagari N., et al. 2006, The J Immunol, 176, 426; Geijtenbeeck T.B., et al. 2000, Cell, 100, 57 ; .Bashirova A. et al., 2001, J.Exp. Med., 193, 671). The clone 111H2.02 anti-DC-SIGN blocks HIVgp120-DC-SIGN interaction and reacts on formalin-fixed tissues (Canard B et al, Immunol Lett. 2011, 135(1-2):165-72).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, van Vliet SJ, Eilering JB, Martin MP, et al. A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection. J Exp Med. 2001 Mar 19;193(6):671-8. PubMed PMID: 11257134. (Free PMC Article available, 5 images available)
  2. Soilleux EJ. DC-SIGN (dendritic cell-specific ICAM-grabbing non-integrin) and DC-SIGN-related (DC-SIGNR): friend or foe? Clin Sci (Lond). 2003 Apr;104(4):437-46. PubMed PMID: 12653690.
  3. Dakappagari N, Maruyama T, Renshaw M, Tacken P, Figdor C, Torensma R, et al. Internalizing antibodies to the C-type lectins, L-SIGN and DC-SIGN, inhibit viral glycoprotein binding and deliver antigen to human dendritic cells for the induction of T cell responses. J Immunol. 2006 Jan 1;176(1):426-40. PubMed PMID: 16365436.
  4. Canard B, Vachon H, Fontaine T, Pin JJ, Paul S, Genin C, et al. Generation of anti-DC-SIGN monoclonal antibodies capable of blocking HIV-1 gp120 binding and reactive on formalin-fixed tissue. Immunol Lett. 2011 Mar 30;135(1-2):165-72. doi: 10.1016/j.imlet.2010.11.002. Epub 2010 Nov 13. PubMed PMID: 21078343.
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Human DC-SIGN.
Species cross-reactivity: positive staining on NIH3T3-transfected LSIGN.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD209 / DC-SIGN (6 products)

Catalog No. Species Pres. Purity   Source  

CD209 / DC-SIGN
CD209 / DC-SIGN Human
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
Densitometry: DC-SIGN Protein [NBP1-46043] - Post-translational modifications result in protein heterogeneity. The densitometry scan, derived from the 2D gel image above, demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification. Expression of these glycoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW. Human Purified
  Novus Biologicals Inc.

Positive controls for CD209 / DC-SIGN (2 products)

Catalog No. Species Pres. Purity   Source  

CD209 293T Cell Transient Overexpression Lysate(Denatured)
CD209 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD209 / DC-SIGN Lysate
Western Blot: DC-SIGN Lysate [NBL1-08921] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CD209
  Novus Biologicals Inc.

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