Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Albumin

R1048BS Albumin antibody

See related secondary antibodies

Search for all "Albumin"

1 ml / €280.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

View Datasheet

Quick Overview

Rabbit anti Bovine Albumin

R1048BS

Product Description for Albumin

Rabbit anti Bovine Albumin.
Presentation: Biotin
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Radioimmunoassay, Western blot / Immunoblot.

Properties for Albumin

Product Category Primary Antibodies
Quantity 1 ml
Synonyms ALB, BSA, HSA, Serum Albumin
Presentation Biotin
Reactivity Bov
Applications Dot, E, ICC/IF, ID, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02769
Immunogen:
Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
280717
Application ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In immunocytochemical and immunohistochemical use for the detection of Albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; in non-isotopic assay methodology (e.g. ELISA) to measure albumin in bovine serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Background Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Concentration 10 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Molar radio:
6.2 moles Biotin per mole of Rabbit IgG.
Purification:
Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2
Preservatives:
None
State:
Lyophilized purified hyperimmune IgG fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
The defined antibody specificity is directed to Albumin as tested against Bovine sera.
In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against Bovine serum and the purified Albumin. 
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results: 
Chicken (-), Dog (+), Goat (++), Guinea Pig (±), Horse (+), Human (+), Monkey (+), Mouse (-), Rabbit (-), Rat (±), Sheep (++), Swine (+).
Species:
Bovine.

Accessory Products

Proteins and/or Positive Controls

Proteins for Albumin (2 products)

Catalog No. Species Pres. Purity   Source  

Albumin
Albumin Bovine Purified > 97.0%
  OriGene Technologies GmbH

Albumin
Albumin Bovine Agarose
  Alpha Diagnostic Intl. Inc.

Recommended Secondary Antibodies for Albumin (9 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Rabbit IgG (H+L chain) antibody
Rabbit IgG (H+L chain) antibody Goat AP Rb E, WB
    OriGene Technologies GmbH

Rabbit IgG (H+L chain) antibody
Rabbit IgG (H+L chain) antibody Goat Biotin Rb E, WB
    OriGene Technologies GmbH

Rabbit IgG (H+L chain) antibody
Rabbit IgG (H+L chain) antibody Goat FITC Rb E, ICC/IF, WB
    OriGene Technologies GmbH

Rabbit IgG (H+L chain) antibody
Rabbit IgG (H+L chain) antibody Goat HRP Rb E, P, WB
    OriGene Technologies GmbH

Rabbit IgG (H+L chain) antibody
Rabbit IgG (H+L chain) antibody Goat TRITC Rb E, F, ICC/IF
    OriGene Technologies GmbH

Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody Donkey PE Rb F
    OriGene Technologies GmbH

Rabbit IgG (H+L chain, adsorbed) antibody
Rabbit IgG (H+L chain, adsorbed) antibody Goat Cy2
    OriGene Technologies GmbH

Rabbit IgG (H+L chain, adsorbed) antibody
Rabbit IgG (H+L chain, adsorbed) antibody Goat Cy3
    OriGene Technologies GmbH

Rabbit IgG (H+L chain, adsorbed) antibody
Rabbit IgG (H+L chain, adsorbed) antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for Albumin
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn