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BM5046 Cytokeratin 9 antibody

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1 ml / €300.00
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Quick Overview

Mouse anti Human Cytokeratin 9 Ks9.70 / Ks9.216

BM5046

Product Description for Cytokeratin 9

Mouse anti Human Cytokeratin 9 Ks9.70 / Ks9.216.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 9

Product Category Primary Antibodies
Quantity 1 ml
Synonyms CK9, Cytokeratin 9, K9, KRT9, Keratin, Keratin-9, Spermatid Perinuclear Ring Manchette Protein K9, Type I cytoskeletal 9
Presentation Supernatant
Reactivity Hu
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone Ks9.70 / Ks9.216
Host Mouse
Isotype cocktail
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35527
Immunogen:
Synthetic peptides [amino acids pos. 450-477 / N-terminal amino acids nos. 4-28] of Human Cytokeratin 9 (MW 62,129).
GeneID:
3857
Add. information Isotypes: IgG1 for Ks9.70 and IgG3 for Ks9.216
Application Immunohistochemistry on Frozen and Paraffin Embedded tissues: 1/20 (PAP/ABC).
For Paraffin sections, microwave treatment and in some cases protease digestion is recommended.
Immunofluorescence: 1/10.
Western blotting: 1/25-1/300 (Chemiluminescence (AP/ECL).
Background Keratin 9 is a type I cytokeratin. It is expressed in the terminally differentiated epidermis of palms and soles. Defects in KRT9 are a cause of epidermolytic palmoplantar keratoderma (EPPK). EPPK is an autosomal dominant disease characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins.
General Readings
  1. Langbein L et al. Molecular characterization of the body site-specific human cytokeratin 9: cDNA cloning, amino acid sequence, and tissue specificity of gene expression. Differentiation 55:57-71 (1994).
  2. Swensson O, Langbein L, McMillan JR, Stevens HP, Leigh IM, McLean WH, et al. Specialized keratin expression pattern in human ridged skin as an adaptation to high physical stress. Br J Dermatol. 1998 Nov;139(5):767-75. PubMed PMID: 9892940.
  3. Mochida K, Rivkin E, Gil M, Kierszenbaum AL. Keratin 9 is a component of the perinuclear ring of the manchette of rat spermatids. Dev Biol. 2000 Nov 15;227(2):510-9. PubMed PMID: 11071770.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Hybridoma Culture Supernatant
Supernatant
Specificity
Specificity:
The antibody cocktail is an excellent tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
The antibody reagent represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia.
Negative tissues include: Muscle, liver and duodenum.
Species:
Human (in Mouse: some background staining).
Gene ID 3857

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 9 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 9
Cytokeratin 9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Cytokeratin 9
Cytokeratin 9 Human
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 9 (1 products)

Catalog No. Species Pres. Purity   Source  

KRT9 overexpression lysate
KRT9 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), adsorbed antibody
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Mouse IgG (H+L chain), adsorbed antibody
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Mouse IgG (H+L chain), adsorbed antibody
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Click here to see all secondary antibodies for Cytokeratin 9
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