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BM563 Cytokeratin 13 antibody

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50 µg / €310.00
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Quick Overview

Mouse anti Bovine, Human, Rat Cytokeratin 13 Ks13.1

BM563

Product Description for Cytokeratin 13

Mouse anti Bovine, Human, Rat Cytokeratin 13 Ks13.1.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 13

Product Category Primary Antibodies
Quantity 50 µg
Synonyms CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13
Presentation Purified
Reactivity Bov, Hu, Rt
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone Ks13.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13646
Immunogen:
Keratin K13 of Mr 54.000 purified from human esophagus.
GeneID:
3860
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western Blot.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: 1/10.
Protease pretreatment improves the performance of this excellent marker in immunohistochemistry with PBS, pH 7.4
Incubation time: 1h at RT, extended with parafin.
Background Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.
General Readings
  1. Dallenbach-Hellweg G, Lang G. Immunohistochemical studies on uterine tumors. I. Invasive squamous cell carcinomas of the cervix and their precursors. Pathol Res Pract. 1991 Jan;187(1):36-43. PubMed PMID: 1709284.
  2. Dockhorn-Dworniczak B, Franke WW, Schröder S, Czernobilsky B, Gould VE, Böcker W. Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. Differentiation. 1987;35(1):53-71. PubMed PMID: 2448179.
  3. Franke, W.W., Moll, R., Achtstätter Th. and Kuhn, C.: Cell typing of epithelia and carcinomas of the female genital tract using cytoskeletal proteins as markers. Banbury Report 21: Viral Etiology of Cervical Cancer, Cold Spring Harbor Laboratory (NY), 121-148 (1986).
  4. Moll R, Achtstätter T, Becht E, Balcarova-Ständer J, Ittensohn M, Franke WW. Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines. Am J Pathol. 1988 Jul;132(1):123-44. PubMed PMID: 2456018. (Free PMC Article available)
  5. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379.
  6. Demirkesen C, Hoede N, Moll R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol. 1995 Dec;22(6):518-35. PubMed PMID: 8835171.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.5% BSA
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in 1 ml dist. water.
Species Reactivity
Species reactivity (tested):
Human, Bovine and Rat.
Specificity
Specificity:
Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13; with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma.
Species:
Human.
Gene ID 3860

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 13 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 2)
Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 1)
Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Positive controls for Cytokeratin 13 (1 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 Lysate
Western Blot: Cytokeratin 13 Lysate [NBL1-12380] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT13
  Novus Biologicals Inc.

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