BP5072 Cytokeratin 2e antibody

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0.1 ml / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Guinea Pig anti Bovine, Human, Mouse Cytokeratin 2e

BP5072

Product Description for Cytokeratin 2e

Guinea Pig anti Bovine, Human, Mouse Cytokeratin 2e.
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 2e

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms CK2e, Cytokeratin-2e, K2e, KRT2, KRT2A, KRT2E, Keratin, Keratin-2, type II cytoskeletal 2 epidermal
Presentation Serum
Reactivity Bov, Hu, Ms
Applications C, ICC/IF, P, WB
Clonality Polyclonal
Host Guinea Pig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35908
Immunogen:
Synthetic peptide (N-terminal aa 2-23) of human keratin K2.
GeneID:
3849
Application Immunoblotting: 1:5000 - 1:10 000; ECL method.
Immunohistochemistry on frozen sections: 1:200.
Immunohistochemistry on paraffin sections: 1:100; 1 h at RT.
Immunohistochemistry on cytological material.
Background Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner.
General Readings
  1. Collin C, Moll R, Kubicka S, Ouhayoun JP, Franke WW. Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation. Exp Cell Res. 1992 Sep;202(1):132-41. PubMed PMID: 1380918.
  2. Swensson O, Langbein L, McMillan JR, Stevens HP, Leigh IM, McLean WH, et al. Specialized keratin expression pattern in human ridged skin as an adaptation to high physical stress. Br J Dermatol. 1998 Nov;139(5):767-75. PubMed PMID: 9892940.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid serum
Serum
Specificity
Specificity:
The antiserum represents an excellent marker to study terminal epidermal differentiation. The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K1). It is also strongly reactive on hyperkeratosis of diverse origin.The mab does not react with palate keratin K76.
Species:
Human, Bovine, Mouse.

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 2e (1 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 2e (full length, N-term HIS tag, transcript variant 1)

Cytokeratin 2e Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Positive controls for Cytokeratin 2e (1 products)

Catalog No. Species Pres. Purity   Source  

KRT2 overexpression lysate

KRT2 overexpression lysate
  OriGene Technologies, Inc.
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